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Updated: Aug 19, 2026
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
Published on: May 6, 2018
[The study of the pro-nucleating activity of bacteria identified in cholesterol gallstones in model bile systems]
Lei-ming Zhu1, Duan Cai, Yuan Lü
1Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Objective:
To explore the relationship of bacteria identified in cholesterol gallstones and gallstone formation.
Methods:
Observe the bacteria activity in model bile and the influence of bacteria on the cholesterol nucleation time (NT).
Results:
(1) Model bile were suitable for the growth of E. coli, Pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, clostridium difficile and Clostridium. Propionibacterium acne grew weakly and the growth of Bacteroides fragilis was restrained in model bile. (2) Only pseudomonas aeruginosa and enTerococcus faecalis could ly shorten the cholesterol nucleation time. (3) With pseudomonas aeruginosa or enTerococcus faecalis added in model bile, the formation of cholesterol crystals presented a progressive course of evolution.
Conclusions:
Pseudomonas aeruginosa and enterococcus faecalis, not propionibacterium acne, have pro-nucleating ability in model bile.
