Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Free fatty acids increase PGC-1alpha expression in isolated rat islets.

Peixiang Zhang1, Chang Liu, Chunni Zhang

  • 1Institute of Mecular Medicine and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.

FEBS Letters
|March 1, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laboratory study on the hygroscopic behavior of external and internal C2-C4 dicarboxylic acid-NaCl mixtures.

Environmental science & technology·2013
Same author

[Arthroscopic reconstruction of anterior cruciate ligament with preservation of the remnant bundle].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2013
Same author

[Anterior cruciate ligament reconstruction with tendon graft enveloped by preserved remnants].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2013
Same author

Genetic and molecular biological characterization of two homologous cheR genes from Leptospira interrogans.

Acta biochimica et biophysica Sinica·2013
Same author

Upregulation of glycoprotein nonmetastatic B by colony-stimulating factor-1 and epithelial cell adhesion molecule in hepatocellular carcinoma cells.

Oncology research·2013
Same author

Effect of implantation of biodegradable magnesium alloy on BMP-2 expression in bone of ovariectomized osteoporosis rats.

Materials science & engineering. C, Materials for biological applications·2013

Free fatty acids (FFA) elevate PGC-1alpha in pancreatic islets, impairing insulin secretion. Inhibiting PGC-1alpha improves this dysfunction, suggesting FFA-induced PGC-1alpha is key to beta-cell issues in hyperlipidemia.

Area of Science:

  • Endocrinology
  • Metabolic Research
  • Molecular Biology

Background:

  • PGC-1alpha (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) is upregulated in diabetic islets, and its overexpression impairs glucose-stimulated insulin secretion (GSIS).
  • The specific metabolic triggers for PGC-1alpha upregulation in beta-cells during pathological conditions remain unclear.

Purpose of the Study:

  • To investigate the distinct effects of chronic hyperlipidemia (free fatty acids, FFA) and hyperglycemia on PGC-1alpha mRNA expression in isolated rat islets.
  • To determine the role of FFA-induced PGC-1alpha in beta-cell dysfunction.

Main Methods:

  • Isolated rat islets were chronically incubated with varying concentrations of oleic/palmitic acid (FFA) or glucose for 72 hours.
  • PGC-1alpha mRNA expression was quantified.

Related Experiment Videos

  • Small interfering RNA (siRNA) was used to inhibit PGC-1alpha.
  • GSIS was measured in islets with and without PGC-1alpha inhibition.

    • Main Results:

    • FFA significantly increased PGC-1alpha mRNA expression in a dose-dependent manner in isolated islets and a mouse beta-cell line (beta TC3).
    • High glucose (25 mM) decreased PGC-1alpha mRNA expression.
    • Inhibition of PGC-1alpha using siRNA ameliorated the impairment of GSIS caused by FFA.

    Conclusions:

    • Hyperlipidemia and hyperglycemia differentially regulate PGC-1alpha expression in pancreatic islets.
    • Elevated PGC-1alpha, induced by FFA, plays a critical role in the beta-cell dysfunction associated with chronic hyperlipidemia.