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TNF-alpha -308G/A and IL-6 -174 G/C polymorphisms in the Turkish pediatric stroke patients
Zeynep Ceren Karahan1, Gülhis Deda, Tansu Sipahi
1Ankara University School of Medicine Division of Pediatric Molecular Pathology and Genetics, Cebeci, 06100, Ankara, Turkey. ckarahan@medicine.ankara.edu.tr
Insights
This study found no significant association between specific gene variations in interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) and the occurrence of stroke in Turkish children. Further research is needed to understand pediatric stroke causes.
Area of Science:
- Genetics
- Pediatric Neurology
- Immunology
Background:
- Pediatric stroke is a significant cause of mortality and disability.
- Proinflammatory cytokines like IL-6 and TNF-alpha are implicated in stroke.
- Functional polymorphisms in IL-6 and TNF-alpha genes may influence cytokine levels and stroke risk.
Purpose of the Study:
- To investigate the association between IL-6 -174 G/C and TNF-alpha -308 G/A polymorphisms and pediatric stroke occurrence.
- To analyze allele and genotype distributions in Turkish pediatric stroke patients and healthy controls.
Main Methods:
- Polymerase Chain Reaction (PCR) and restriction endonuclease analysis were used.
- Investigated 86 pediatric stroke patients (0-14 years) and 83 healthy adult controls.
- Examined IL-6 -174 G/C and TNF-alpha -308 G/A polymorphisms.
Main Results:
- No significant differences were observed in allele distribution between patient and control groups.
- Allele frequencies for both polymorphisms did not differ significantly.
- Combined genotype distributions showed no significant association with stroke occurrence.
Conclusions:
- The studied IL-6 and TNF-alpha gene polymorphisms are not associated with the occurrence of stroke in the Turkish pediatric population.
- These specific genetic variations do not appear to be risk factors for pediatric stroke in this cohort.
Introduction:
Introduction: Stroke is an important cause of mortality and morbidity in the pediatric age group. There is increasing evidence on the role of proinflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in the occurrence and outcome of stroke. These proinflammatory cytokines carry functional polymorphisms (IL-6 -174 G/C and TNF-alpha -308 G/A) in their promoter regions, affecting their transcription rate and plasma cytokine levels. The aim of this study was to investigate the association of these polymorphisms with the occurrence of stroke in the Turkish pediatric stroke patients.
Material And Methods:
Eighty six arterial stroke patients (aged between 0 and 14) and 83 healthy unrelated adult controls without personal or family history of stroke were investigated by PCR and restriction endonuclease analysis for IL-6 -174 G/C and TNF-alpha -308 G/A polymorphisms.
Results:
The allele distribution, allele frequencies, and combined distribution of genotypes for both polymorphisms did not differ significantly between control and patient groups.
Conclusions:
The two polymorphisms did not associate with the occurrence of stroke in our study group.
