Related Experiment Video
Updated: Aug 19, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
Effect of molybdate on methanogenic and sulfidogenic activity of biomass
1Department of Civil Engineering, National Institute of Technology, Kurukshetra 136 119, India.
Abstract:
The effect of molybdate, a sulfate analog, on the total methanogenic activity (TMA) and total sulfidogenic activity (TSA) of biomass metabolizing synthetic sucrose based substrate containing sulfate was investigated in batch assays. In Phase I of the study, TMA and TSA were assessed twice for four feed changes at a chemical oxygen demand to sulfate (COD/SO(4)(2-)) ratio of 3.5. In Phase II, long-term experiments were conducted for 10-13 feed changes with varying chemical oxygen demand (COD) concentration, sulfate concentration, COD/SO(4)(2-) ratio, molybdate dose and biomass with different growth histories. Assays with 3mM molybdate showed TSA inhibition over 85%. Dose dependency was observed for sulfate concentration, COD/SO(4)(2-) ratio, and biomass history. The minimum concentration that gave over 93% TSA inhibition was 0.25 mM. However, intermediate concentrations of molybdate inhibited methane producing bacteria (MPB) activity. TMA stimulation was observed at 0.75-2.0 mM molybdate.
More Related Videos
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
07:31Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
Published on: September 6, 2024
Related Concept Videos
Microbes and Methanogenesis
Microbial Mats
Microbial Interactions: Mutualism
Microbial Corrosion
Microbial Wastewater Treatment
Microbial Bioremediation of Uranium