An immunohistochemical analysis to evaluate an inverse correlation between Runx2/Cbfa1 and NF kappa B in human
V B Andela1, F Siddiqui, A Groman
1The James P Wilmot Cancer Center, University of Rochester Medical Center, 601 Elmwood Avenue Box 665, Rochester, NY 14642, USA. v_andela@yahoo.com
Background:
Dominant negative inhibition of nuclear factor kappa B (NF kappa B) signalling activity in a human osteosarcoma cell line (Saos2) results in malignant reversion and the induction of the osteoblast differentiating transcription factor, Runx2/Cbfa1. This observation suggests that there is an inverse relation between a transcription factor associated with malignant progression and chemoresistance (NF kappa B) and an osteoblast differentiating transcription factor (Runx2/Cbfa1).
Aims:
To assess and correlate Runx2/Cbfa1 and NF kappa B (p65) immunoreactivity in human osteosarcoma.
Methods:
Runx2/Cbfa1 and NFkappaB (p65) immunoreactivity was assessed on 11 paraffin wax embedded archival specimens of human primary osteosarcoma by standard immunohistochemical methods and scored on a scale of 0-3. A Pearson correlation analysis between Runx2/Cbfa1 and NF kappa B (p65) scores was established.
Results:
Runx2/Cbfa1 was expressed constitutively in all pathology specimens of human osteosarcoma. Of note, a chondroblastic osteosarcoma showed the highest Runx2/Cbfa1 immunoreactivity. A Pearson correlation did not support an inverse correlation between Runx2/Cbfa1 and NF kappa B (p65) scores (r = 0.57) in human osteosarcoma.
Conclusion:
Runx2/Cbfa1 immunoreactivity does not inversely correlate with NF kappa B immunoreactivity, and thus cannot serve as an indirect measure of NF kappa B activity or an independent predictive or prognostic indicator.
Insights
Nuclear factor kappa B (NF kappa B) and Runx2/Cbfa1 expression do not inversely correlate in osteosarcoma. Therefore, Runx2/Cbfa1 cannot predict NF kappa B activity or serve as a prognostic indicator in this cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Nuclear factor kappa B (NF kappa B) signaling inhibition in osteosarcoma cells induced malignant reversion and Runx2/Cbfa1 expression.
- This suggests an inverse relationship between NF kappa B (malignancy, chemoresistance) and Runx2/Cbfa1 (osteoblast differentiation).
Purpose of the Study:
- To evaluate and correlate Runx2/Cbfa1 and NF kappa B (p65) protein expression in human osteosarcoma tissues.
- To determine if Runx2/Cbfa1 can serve as an indirect marker for NF kappa B activity.
Main Methods:
- Immunohistochemical analysis of Runx2/Cbfa1 and NF kappa B (p65) in 11 archival human osteosarcoma specimens.
- Scoring of immunoreactivity on a 0-3 scale and statistical analysis using Pearson correlation.
Main Results:
- Runx2/Cbfa1 was constitutively expressed in all osteosarcoma samples, with highest levels in chondroblastic osteosarcoma.
- Pearson correlation analysis (r = 0.57) did not support an inverse correlation between Runx2/Cbfa1 and NF kappa B (p65) scores.
Conclusions:
- Runx2/Cbfa1 immunoreactivity is not inversely correlated with NF kappa B immunoreactivity in human osteosarcoma.
- Runx2/Cbfa1 cannot be used as an indirect measure of NF kappa B activity or as an independent predictive/prognostic indicator.
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