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Vasorelaxant effect of resin-based, single-bottle dentin bonding systems
Worku Abebe1, David H Pashley, Fred A Rueggeberg
1Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1128, USA. wabebe@mail.mcg.edu
Journal of Endodontics
|March 1, 2005
Summary
Single-bottle dentin bonding agents like 3M Single Bond and Prime & Bond NT cause blood vessels to relax, potentially increasing bleeding when applied to dental pulp exposures.
Area of Science:
- Biomaterials Science
- Dental Materials
- Vascular Biology
Background:
- Single-bottle dentin bonding systems are widely used in dentistry.
- Accidental or deliberate pulp exposure necessitates understanding the effects of these bonding agents on pulpal tissues.
Purpose of the Study:
- To evaluate the vascular effects of 3M Single Bond (3MSB) and Prime & Bond NT (PBNT) on rat aortic ring preparations.
- To determine if these bonding agents induce vasorelaxation and elucidate the underlying mechanisms.
Main Methods:
- Utilized rat aortic ring preparations to assess the vascular responses to 3MSB and PBNT.
- Conducted concentration-dependent relaxation assays on both intact and endothelium-denuded aortic rings.
Main Results:
- Both 3MSB and PBNT induced concentration-dependent, endothelium-dependent and -independent vasorelaxations.
- Endothelium-dependent relaxation was linked to nitric oxide release, without prostanoid generation or KATP channel activation.
- 3MSB showed greater effects on endothelium-denuded tissues at low concentrations compared to PBNT.
Conclusions:
- 3MSB and PBNT interfere with vascular function by inducing vasorelaxation through smooth muscle and endothelial mechanisms, including nitric oxide release.
- This vasorelaxant effect may contribute to increased bleeding when bonding agents contact pulp exposures.
- Differences exist in the vascular actions of 3MSB and PBNT.