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A functional interaction between ATF7 and TAF12 that is modulated by TAF4

Pierre-Jacques Hamard1, Rozenn Dalbies-Tran, Charlotte Hauss

  • 1Ecole Supérieure de Biotechnologie de Strasbourg, Université Louis Pasteur, Parc d'innovation, UMR7100 CNRS-ULP, Bd. Sebastien Brant-BP10413, 67412 Strasbourg, Illkirch Cedex, France.

Oncogene
|March 1, 2005
PubMed

Insights

Activating transcription factor 7 (ATF7) interacts with TAF12, a component of the general transcription factor TFIID. This interaction enhances ATF7

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Transcription Factors

Background:

  • Activating transcription factor 7 (ATF7) is a versatile transcription factor.
  • ATF7 interacts with adenovirus E1a, Jun, Fos, and JNK2.
  • hsTAF12 is a component of the general transcription factor TFIID.

Purpose of the Study:

  • To investigate the functional relationship between ATF7 and hsTAF12.
  • To determine if TAF12 is a functional partner of ATF7.

Main Methods:

  • Overexpression studies to assess transcriptional activation.
  • Direct interaction assays.
  • Chromatin immunoprecipitation (ChIP) to confirm in vivo interaction.
  • Competitive inhibition assays using TAF4.

Main Results:

  • Overexpression of hsTAF12 potentiates ATF7-induced transcriptional activation via direct interaction.
  • ATF7 and TAF12 interact on an ATF7-responsive promoter in vivo.
  • TAF4 competitively inhibits TAF12-dependent transcriptional activation.
  • Both TAF12 isoforms interact with ATF7's activation region via their histone-fold domain.
  • Only the larger hsTAF12-1 isoform mediates transcriptional activation through its N-terminal region.

Conclusions:

  • TAF12 is a functional partner of ATF7, enhancing its transcriptional activity.
  • The interaction between ATF7 and TAF12 is crucial for gene regulation.
  • Specific isoforms and domains of TAF12 play distinct roles in mediating ATF7 function.

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