Related Experiment Video

Updated: Aug 19, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

Targeting against epidermal growth factor receptors. Cellular processing of astatinated EGF after binding to cultured

Anna Orlova1, Anna Sjöstrom, Ondrej Lebeda

  • 1Division of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden. Anna.Orlova@bms.uu.se

Anticancer Research
|March 2, 2005
PubMed
Abstract

Insights

Astatine-211 labeled EGF showed longer cellular retention than iodine-125, suggesting potential benefits for targeted radionuclide therapy in cancer treatment.

Area of Science:

  • Nuclear medicine
  • Radiopharmaceutical chemistry
  • Oncology

Background:

  • Alpha-emitting nuclide 211At is promising for radionuclide therapy.
  • Targeting biomolecules like EGF are overexpressed in malignancies.
  • Limited data exists on cellular processing of astatinated agents.

Purpose of the Study:

  • To investigate the cellular processing of astatinated EGF ([211At]-benzoate-EGF).
  • To compare its behavior with iodine-125 labeled EGF ([125I]-benzoate-EGF).

Main Methods:

  • Indirect astatination of EGF using [211At]-benzoate.
  • Cellular processing study in A-431 carcinoma cells.
  • Comparative analysis with [125I]-benzoate-EGF.

Main Results:

  • Astatine label ([211At]) exhibited a longer biological half-life (3.5 h) than iodine ([125I]) (1.5 h).
  • Longer retention of internalized astatine radioactivity contributed to the increased half-life.
  • Maximum accumulation of astatine occurred later (4-6h) compared to iodine (2-4h), indicating slower excretion.

Conclusions:

  • The extended cellular retention of astatine suggests its potential advantage for radionuclide therapy.
  • Further research into astatinated agents for cancer treatment is warranted.

More Related Videos

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy09:16

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

Published on: September 11, 2015

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure09:38

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

Published on: August 11, 2017

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...