Related Experiment Video
Updated: Aug 19, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Potential fungicidal effect of voriconazole against Candida spp
M Carmen Rubio1, Inmaculada Ramírez de Ocáriz, Joaquina Gil
1Microbiology, Hospital Clínico Universitario Lozano Blesa, Domingo Miral s/n, Zaragoza 50009, Spain. mcrubio@unizar.es
Abstract:
In vitro fungistatic and fungicidal activities of voriconazole were evaluated against 114 isolates of Candida spp. MICs were determined using the NCCLS M27-A2 broth microdilution method. Minimum fungicidal concentrations (MFCs) were defined as the lowest drug concentrations that yielded < or = 5 colonies (> or = 98% killing activity). The fungicidal activity could be only evaluated against 45.6% of the isolates (52 of 114 isolates) because trailing growth occurred, and 30 of these 52 isolates (57.69%) showed MFC values < or = 1 mg/l. There was little or no fungicidal activity against C. albicans and C. tropicalis. In contrast, all C. krusei were killed by 1-2 mg/l voriconazole. In conclusion, our data show that the fungicidal effect of voriconazole against Candida spp. is species dependent.
Related Concept Videos
Candidiasis
Antifungal Agents
Cryptococcal Meningitis
Antimicrobial Effectiveness
Fungal Group Zygomycota

