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Detection and Isolation of Apoptotic Bodies to High Purity
12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

An antigen recognized on cells in apoptosis detected by monoclonal antibody 2E12

G Stibríková1, I Marinov, P Stöckbauer

  • 1Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion, Prague 2, 12820 Czech Republic.

Neoplasma
|March 2, 2005
PubMed

Insights

Monoclonal antibody 2E12 identifies a marker on dying cells. This new marker can help distinguish between living, apoptotic, and necrotic cells in laboratory studies.

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • Monoclonal antibodies are crucial tools in biological research.
  • Understanding cell death pathways is vital in diseases like leukemia.
  • Identifying specific markers for cell death stages aids research.

Purpose of the Study:

  • To characterize the novel monoclonal antibody 2E12.
  • To investigate the utility of 2E12 as a marker for apoptosis and necrosis.
  • To assess 2E12's potential in distinguishing cell populations in vitro.

Main Methods:

  • Immunization of mice with MOLM-7 cells to generate monoclonal antibody 2E12.
  • Induction of apoptosis in human hematopoietic cell lines (JURKAT, HPB-ALL, RC2A, MOLM-7) using anti-Fas antibody 7C11.
  • Two-color immunofluorescence and flow cytometry analysis using 2E12, annexin V-FITC, and propidium iodide (PI).
  • Two-color fluorescence microscopy.

Main Results:

  • Monoclonal antibody 2E12 reactivity increased over time in apoptotic cells, partially correlating with annexin V-FITC binding.
  • 2E12 positivity was observed on a subset of annexin V-positive cells.
  • 2E12 binding did not induce apoptosis or affect annexin V binding.
  • Antibody 2E12 appears to detect an antigen expressed on cells undergoing death.

Conclusions:

  • Antibody 2E12 shows promise as a novel marker for identifying cells in the process of dying.
  • It can aid in differentiating between viable, apoptotic, and necrotic cell populations in vitro.
  • Further research can explore 2E12's application in various cell death studies.

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