Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

her9 promotes floor plate development in zebrafish.

Andrew J Latimer1, Jimann Shin, Bruce Appel

  • 1Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235-1634, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 2, 2005
PubMed
Summary

Her9, a gene in hairy/Enhancer of split family, regulates vertebrate embryonic midline development. It inhibits notochord formation and promotes floor plate specification, acting as a downstream effector of Nodal signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zebrafish oligodendrocyte lineage cells use the postsynaptic protein Gephyrin to myelinate GABAergic axons and limit myelin sheath growth.

Nature communications·2026
Same author

Regulation of the yolk microtubule and actin cytoskeleton by Dachsous cadherins during zebrafish epiboly.

Developmental biology·2026
Same author

Rocuronium Dose and First-Attempt Intubation Success in the Critically Ill: Secondary Analysis of Two Multicenter Trials.

American journal of respiratory and critical care medicine·2026
Same author

Prehospital Resuscitation with Type O Whole Blood for Trauma and Hemorrhage.

The New England journal of medicine·2026
Same author

Video versus direct laryngoscopy for tracheal intubation in trauma: A secondary analysis of the DEVICE trial.

The journal of trauma and acute care surgery·2026
Same author

Transient activation of potent progenitor cells is required for spinal cord regeneration.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Vertebrate embryonic midline structures like the notochord and floor plate are crucial for patterning the nervous system and other tissues.
  • Notch signaling is a key regulator of cell fate specification in these midline precursor cells.
  • Understanding the molecular mechanisms downstream of Notch signaling is essential for comprehending early vertebrate development.

Purpose of the Study:

  • To investigate the role of potential Notch target genes in vertebrate embryonic midline development.
  • To elucidate the function of the her9 gene in the specification of notochord and floor plate.
  • To determine the signaling pathways regulating her9 expression in midline precursor cells.

Main Methods:

  • Expression analysis of her9 in midline precursors during gastrulation.

Related Experiment Videos

  • Functional studies to assess the impact of her9 on notochord and floor plate development.
  • Investigation of the relationship between Notch signaling, Nodal signaling, and her9 expression.

    • Main Results:

    • Midline precursors express her9, a gene from the hairy/Enhancer of split family.
    • Her9 inhibits notochord development while promoting floor plate specification.
    • Her9 expression in floor plate cells is regulated by Nodal signaling, not Notch signaling.

    Conclusions:

    • Her9 plays a critical role in differentiating between notochord and floor plate cell fates.
    • Nodal signaling, rather than Notch signaling, is the primary pathway regulating her9 for floor plate specification.
    • This study reveals a novel regulatory mechanism in vertebrate embryonic midline patterning.