[Determination of icariin in Epimedium koreanum nakai by high performance liquid chromatography]

An HPLC method for determination of icariin in epimedium koreanum nakai was investigated. The results showed that this method was simple, specific and accurate. The recovery was 97.6% and relative standard deviation was 1.2%. About 0.5 g of dried powdered crude drug was weighed accurately, placed in 25 mL of the mobile phase and refluxed on a water-bath for 30 min. After cooling, the solution was decanted. The residue was refluxed twice with 10 mL of the mobile phase. The extract were placed in a 50 mL volumetric flask and diluted to 50 mL with the mobile phase. A 3 microL of the solution was injected into the HPLC system. Linear calibration graphs for icariin were obtained for the concentration ranges of 0.08-0.4 microg. HPLC conditions were used was Shimadzu LC-6A with UV spectrometric detector SPD-6AV; at 270 nm and auto injector SIL-6A. Water-acetonitrile (70:30) as the mobile phase, Shim-pack CLC-ODS column (6.0 mm x 150 mm).