[Determination of multicomponent contents in anticold solution by high performance liquid chromatography]

A high performance liquid chromatographic method has been successfully used to separate and determine the six drugs in an anticold solution. In the anticold solution, potassium guaiacol sulfonate, acetaminophen, D,L-methylephedrine, and caffeine were analyzed with a column of Zorbax ODS, by using the mobile phase of MeOH-0.05 mol/L KH2PO4-H3PO4 (250 : 750 : 0.5) and UV detector at 214 nm, dextromethorphan HBr and chlorpheniramine maleate were analyzed with the same column, by usingthe mobile phase of MeOH-H2O-HsPO4-SLS (80 : 20 : 0.5 : 0.2) and UV detector at 260 nm. This method is proved to be very effective to separate the six drugs mentioned above. For potassium guaiacol sulfonate, acetaminophen, D,L-methylephedrine, caffeine, dextromethorphan HBr and chlorpheniramine, the response values were linear between 1.0-10 microg, 5.0-50 microg, 0.25-2.5 microg, 0.5-5 microg, 0.25-2.5 microg, 0.05-0.5 microg respectively and the recoveries were 100.53% +/- 1.12%, 100.19% +/- 0.58%, 99.4% +/- 1.60%, 99.55% +/- 1.01%, 99.37% +/- 1.52%, 99.01% +/- 1.20% (n=5) respectively.