Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Capillary isoelectric focusing method and its applications].

X Liu1, Q Wang, L Ma

  • 1Beijing Institute of Radiation Medicine, Beijing, 100850.

Se Pu = Chinese Journal of Chromatography
|March 3, 2005
PubMed
Summary

Capillary isoelectric focusing (CIEF) offers effective separation of complex biological samples. This review evaluates CIEF methods, recommending coated capillaries and discussing applications in protein isoform analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[In vitro inhibitive effects of Tripterygium wilforii on NO production, iNOS activity, and iNOS-mRNA expression in chondrocyrtes of patients with rheumatoid arthritis].

Zhonghua yi xue za zhi·2002
Same author

[Influence of fluid shear stress on the expression of monocyte chemotactic protein-1 by vascular endothelial cells].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2002
Same author

[Study of pefloxacin concentration in blood and sputum in the aged pneumonia patients with impairment of renal function].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2002
Same author

[A retrospective analysis of autogenous alveolar bone grafting in clefts of the lip and palate].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2002
Same author

[Effect of age on mineralization expression of periodontal ligament cell clones].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2002
Same author

Role of angiotensin II in glomerular injury.

Seminars in nephrology·2001

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Capillary isoelectric focusing (CIEF) is a powerful separation technique for charged molecules.
  • Optimizing CIEF requires understanding separation mechanisms and electroosmotic flow.
  • Various methods and challenges exist for sample determination in CIEF.

Purpose of the Study:

  • To review and evaluate different methods for performing capillary isoelectric focusing (CIEF).
  • To discuss key aspects of CIEF, including separation mechanisms, electroosmosis manipulation, mobilization, and detection.
  • To present applications of CIEF in analyzing specific biological molecules.

Main Methods:

  • Review of existing literature on capillary isoelectric focusing (CIEF) with 64 references.
  • Evaluation of different CIEF techniques and capillary coatings.

Related Experiment Videos

  • Discussion of electroosmotic flow manipulation and sample mobilization strategies.

    • Main Results:

    • Non-cross-linked polyacrylamide coated capillaries are recommended for CIEF.
    • Dynamical coating with cellulose polymers can enable sample detection in untreated columns via residual electroosmotic flow.
    • CIEF is applicable for separating challenging samples like transferrin isoforms, human Hb variants, and t-PA glycoforms.

    Conclusions:

    • Capillary isoelectric focusing (CIEF) is a versatile technique with optimized methods for various applications.
    • Proper capillary coating and electroosmotic flow management are crucial for successful CIEF separations.
    • CIEF demonstrates significant utility in the characterization of protein variants and isoforms.