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Updated: Aug 19, 2026
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Fast analysis of glycosaminoglycans by microchip electrophoresis with in situ fluorescent detection using ethidium
Yu-ki Matsuno1, Mitsuhiro Kinoshita, Kazuaki Kakehi
1Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashi-osaka 577-8502, Japan.
Abstract:
We analyzed some glycosaminoglycans and natural and artificial acidic polysaccharides using microchip electrophoresis in the buffer containing ethidium bromide, and found that they were successfully separated and detected within 150 s with comparable sensitivity with that of conventional electrophoresis using cellulose acetate membrane. We applied the technique to the analysis of glycosaminoglycans in pharmaceutical preparations and also in cultured cancer cells. Rapidness and easy operation of the proposed technique are quite useful for routine analysis of glycosaminoglycans.
