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A method for the rapid automated assessment of olfactory function.

Steven L Youngentob1

  • 1Department of Neuroscience and Physiology, SUNY Upstate Medical University, Institute for Human Performance, 750 East Adams Street, Syracuse, NY 13210. USA. youngens@upstate.edu

Chemical Senses
|March 3, 2005
PubMed
Summary

This study introduces an automated method to screen animal odor responsivity by analyzing breathing patterns, offering a rapid, non-invasive technique for biological research. The developed

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Area of Science:

  • Neuroscience
  • Animal Behavior
  • Physiology

Background:

  • Assessing olfactory perception in animals is crucial for understanding sensory processing and behavior.
  • Existing methods for evaluating odor responsivity often require extensive training or are not high-throughput.
  • There is a need for objective, automated, and rapid screening tools for olfactory function in research animals.

Purpose of the Study:

  • To develop and validate a fully automated, high-throughput screening strategy for assessing odor responsivity in experimentally altered animals.
  • To establish a reliable and operationally defined measure of olfactory-driven breathing responses.
  • To demonstrate the method's efficacy in discriminating between different experimental groups and phenotypes.

Main Methods:

Related Experiment Videos

  • Utilized whole-body plethysmography to measure 14 respiratory parameters in response to air and odorant stimuli (propanol).
  • Developed a 'Sniffing Index' by modeling stimulus-induced sniffing behaviors into a single univariate measure.
  • Applied epidemiologic screening principles for operational evaluation of unknown animals.

    • Main Results:

    • The automated procedure successfully discriminated between control and odor-aversion-conditioned mice.
    • Significantly differentiated between a mutant phenotype (OMP-null) and its background strain based on olfactory response.
    • The 'Sniffing Index' provided a robust measure of reflexive sniffing behavior.

    Conclusions:

    • The developed automated screening strategy offers a rapid, objective, and non-invasive method for evaluating animal odor responsivity.
    • This technique is valuable for high-throughput screening in genetic and physiological research, particularly in neuroscience and behavior.
    • The method provides a validated operational procedure for assessing olfactory function in diverse animal models.