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Published on: April 22, 2016
Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis
Hideshi Yanase1, Koji Nozaki, Kenji Okamoto
1Department of Biotechnology, Tottori University, Tottori, Tottori, 680-8552, Japan. yanase@bio.tottori-u.ac.jp
Abstract:
To confer the ability to ferment cello-oligosaccharides on the ethanol-producing bacterium, Zymomonas mobilis, the beta-glucosidase gene from Ruminococcus albus, tagged at its N-terminal with the 53-amino acid Tat signal peptide from the periplasmic enzyme glucose-fructose oxidoreductase from Z. mobilis, was introduced into the strain. The tag enabled 61% of the beta-glucosidase activity to be transported through the cytoplasmic membrane of the recombinant strain which then produced 0.49 g ethanol/g cellobiose.
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