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Updated: Aug 19, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Pycnogenol stimulates lipolysis in 3t3-L1 cells via stimulation of beta-receptor mediated activity
Miyako Mochizuki1, Noboru Hasegawa
1Department of Health and Nutrition, School of Health and Human Life, Nagoya Bunri University, Inazawa, Japan.
Abstract:
The influence of pycnogenol on the glycerol released into the medium in fully differentiated 3T3-L1 cells was studied. After incubation for 2 h, pycnogenol stimulated glycerol release in a dose-dependent manner. Pretreatment with the beta-receptor antagonist, propranolol, significantly reduced pycnogenol-induced lipolysis in a dose-dependent manner. When fully differentiated 3T3-L1 cells were incubated with pycnogenol, the cyclic adenosine monophosphate content significantly increased. These data suggested that pycnogenol has strong lipolytic effects via stimulation of beta-receptor mediated activity.
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