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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2
Dianah Barrett1, Catherine Leimkuhler, Lan Chen
1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
Journal of Bacteriology
|March 4, 2005
Abstract:
We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.

