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Updated: Aug 19, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Radical species in DNA strand-cleavage caused by dihydropyrazines
Nobuhiro Kashige1, Toru Takeuchi, Shigenobu Matsumoto
1Faculty of Pharmaceutical Sciences, Fukuoka University, Fokuoka, Japan.
Abstract:
Dihydropyrazines (DHPs), which generate hydroxyl and carbon-centered radicals, cleaved DNA single-strand. It is new knowledge that DHPs were recently determined to produce 8-hydroxydeoxyguanosine. The remarkable increase in the DNA strand-cleavage activity upon the addition of Cu2+ suggests that the primary reactive species is carbon-centered radicals rather than the hydroxyl radical generated the initiation reaction. This proposal was in fair agreement with of the observed carbon-centered radical signal intensity, but an effect was not observed with the increase in the hydroxyl radical signal intensity.
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