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Related Experiment Videos

Improving surgical wound healing with basic fibroblast growth factor after radiation.

David B Hom1, Gretchen M Unger, Kerri J Pernell

  • 1Division of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology-Head and Neck Surgery, University of Minnesota School of Medicine and, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

The Laryngoscope
|March 4, 2005
PubMed
Summary

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Radiation significantly reduces basic fibroblast growth factor (bFGF) in skin, impairing wound healing. Supplemental intravenous bFGF improved irradiated skin flap viability and reduced injury in a porcine model.

Area of Science:

  • Regenerative Medicine
  • Wound Healing Research
  • Radiation Oncology

Background:

  • Delayed wound healing in patients with prior irradiation is a significant clinical challenge.
  • Radiation exposure is known to impair tissue repair processes.
  • The role of basic fibroblast growth factor (bFGF) in irradiated tissue healing requires further elucidation.

Purpose of the Study:

  • To investigate if radiation decreases basic fibroblast growth factor (bFGF) production in skin.
  • To determine if supplemental bFGF can enhance soft tissue healing in irradiated surgical sites.
  • To assess the impact of bFGF administration routes and hyperbaric oxygen on healing outcomes.

Main Methods:

  • An experimental study utilizing a porcine skin flap model subjected to orthovoltage radiation (1,300 cGy).

Related Experiment Videos

  • Quantification of bFGF messenger RNA (mRNA) using reverse transcription-polymerase chain reaction (RT-PCR) in irradiated versus non-irradiated skin.
  • Administration of supplemental bFGF intravenously or intracuticularly preoperatively, with some groups receiving hyperbaric oxygen, followed by skin flap creation and monitoring for 2 weeks.

    • Main Results:

    • Radiation significantly increased endothelial cell apoptosis by 650% and reduced bFGF mRNA by 75% in porcine skin.
    • Supplemental intravenous bFGF significantly improved skin flap viability by 25% and reduced gastrointestinal side effects by 50% compared to controls.
    • Intracuticular bFGF administration, with or without hyperbaric oxygen, did not significantly improve flap survival or vascularity.

    Conclusions:

    • Reduced local bFGF levels play a crucial role in the delayed healing of irradiated wounds.
    • Radiation diminishes bFGF production by significantly decreasing its mRNA in irradiated tissues.
    • Intravenous bFGF shows potential in mitigating radiation-induced soft tissue injury and improving surgical wound healing in preclinical models.