Antibody formation to dog pulp tissue altered by AH-26 sealer via the root canal

Pulp tissue from three experimental dogs was tested for its antigenicity before and after incubation with AH-26 sealer. identical to Reactions to Arthus skin tests showed an increased response to pulp that had been incubated in AH-26, washed, and then injected, as compared with the material alone. Hemagglutinating antibody titers (1/500 to 1/800) were obtained from pulp tissue altered by AH-26. Inhibition tests showed the apecificity of the antibody to be at a dilution titer of 1:400. Therefore, a mixture of AH-26 sealer altered dog pulp tissue and rendered it antigenically active, and a specific humoral response was produced.