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Published on: January 13, 2016
[Expression of Interleukin-8 in KB cells infected with Candida albicans]
Ling Zhang1, Zhao-Yuan Wang, Ming Zhong
1College of Stomatology, China Medical University. Shenyang 110002,Liaoning Province, China. zhanglingab@yahoo.com
Purpose:
To investigate the change of interleukin-8 (IL-8) in oral epithelial cells (KB cells) infected with Candida albicans.
Methods:
Two-site ELISA and RT-PCR were applied to determine the levels of IL-8 protein or mRNA expression in the KB cells induced by hyphae, yeast, killed Candida albicans and medium respectively. The unpaired Student's t test was used to analyse the data.
Results:
Candida albicans induced the KB cells secrete more IL-8. The hyphae, yeast, killed Candida albicans and control group marks of RT-PCR were 180.23, 186.36, 143.41 and 85.57 respectively, calculated using a two-tailed test, P<0.05, was considered to be significant, but the medium group (mark 80.87) had no significant change. The tendency of the IL-8 protein secreted by KB cells increased with the time but reduced when Candida albicans was more.
Conclusions:
Candida albicans is responsible for the production of IL-8 secreted by oral epithelial cells which can be expected to play an important role in the infection and defection with the local oral mucosa.
