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Updated: Aug 19, 2026

Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice
Published on: September 17, 2015
Volume-weighted mean glomerular volume in spontaneously hypertensive rats treated with different doses of
Leila M M Pereira1, Danielle L Querido, Agata C M Madeira
1Laboratory of Morphometry and Cardiovascular Morphology, Biomedical Center, State University of Rio de Janeiro (UERJ), RJ, Brazil. lmaria@uerj.br
Objectives:
Ascertain alterations in volume-weighted glomerular volume in monotherapy of spontaneously hypertensive rats with different doses of spironolactone.
Material And Methods:
We have studied young adult male spontaneously hypertensive rats (SHR) 20 weeks old, divided in 4 groups of 5 animals each: Controls, spironolactone lower dose (5mg/kg/day), spironolactone average dose (10mg/kg/day), and spironolactone higher dose (30mg/kg/day) dissolved in drinking water (for 12 weeks). Serum albumin, blood urea nitrogen (BUN), and creatinine, and the volume-weighted mean glomerular volume (VWGV) were measured.
Results:
Common progress of blood pressure (BP) in young adult SHR was greatly altered by spironolactone treatment, by either attenuation or reversion of BP increasing tendency. Biochemical evaluation of kidney function indicated normal levels for albumin and creatinine, and high levels for BUN in SHR in all groups. Thickened intrarenal arteries and venous stasis were observed in all groups. Treated SHR spironolactone average and higher doses glomeruli presented smaller and more regular profiles; glomerular hypertrophy with glomerular tuft gross disarray in controls and treated SHR spironolactone lower dose was observed. VWGV was significantly greater in control and treated SHR spironolactone lower dose than in treated SHR spironolactone average and higher doses.
Conclusions:
Monotherapy with spironolactone may affect glomerular size and shape in a dose-dependent way; spironolactone showed a significant effect in preserving VWGV, and may be used associated with other drugs in antihypertensive therapy to prevent secondary effects of hypertension in the kidney.
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