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Updated: Aug 19, 2026
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
Antibacterial activity of gentamicin-bonded gelatin-sealed polyethylene terephthalate vascular prostheses
G Ginalska1, M Osinska, A Uryniak
1Department of Biochemistry, M. Curie-Sklodowska University, 3 Maria Curie-Sklodowska Square, 20-031 Lublin, Poland. ginal@hermes.umcs.lublin.pl
Objectives:
To create an antibiotic-modified vascular prosthesis with a prolonged bactericidal activity, susceptible to endothelialisation.
Methods:
We used a covalent method of gentamicin sulphate immobilisation to polyethylene terephthalate prosthesis sealed with gelatin. Antibacterial activity was assayed in Luria-Bertani medium against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa strains. Prosthesis endothelialisation was performed using bovine aorta endothelial cells (BAEC).
Results:
Gentamicin was bound to vascular prostheses in the amount of 12g per kg of prosthesis. Ninety-seven percent of antibiotic bound in covalent way and remained on the biomaterial for at least 30 days during shaking in PBS solution. Gentamicin-modified prostheses exerted bactericidal or bacteriostatic effect on growth of clinical and reference bacterial strains, prevented biofilm formation and were highly susceptible to endothelialisation. BAEC viability exceeded 90%, which indicated that gentamicin-vascular prostheses were not toxic for these cells.
Conclusions:
Covalent gentamicin immobilisation resulted in effective antibacterial protection of vascular prostheses against clinical and reference strains of S. aureus, E. coli and P. aeruginosa and allowed for a strong adherence of endothelial cells to antibiotic-modified prostheses.