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Related Experiment Videos

Cell culture-based models for intestinal permeability: a critique.

Praveen V Balimane1, Saeho Chong

  • 1Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb, Princeton, NJ 08543, USA. praveen.balimane@bms.com

Drug Discovery Today
|March 8, 2005
PubMed
Summary

Current drug discovery models for intestinal permeability combine fast, less accurate computational and in vitro methods with slow, more accurate in vivo studies. This analysis reviews cell culture models for compound screening and future trends.

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Area of Science:

  • Pharmacokinetics and Drug Discovery
  • Cell Biology and In Vitro Modeling

Background:

  • Intestinal permeability is crucial for drug absorption and efficacy.
  • Current compound screening relies on a mix of in silico, in vitro, and in vivo models.
  • Cell-based permeability assays are widely adopted in drug discovery.

Purpose of the Study:

  • To objectively analyze the effectiveness of cell culture models in permeability screening.
  • To discuss emerging trends and future directions for cell-based permeability models in drug discovery.

Main Methods:

  • Review and analysis of existing cell-based permeability models.
  • Comparative assessment of in silico, in vitro, and in vivo model predictivity.
  • Discussion of industry-standard practices in compound permeability screening.

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Main Results:

  • Cell culture models are the industry standard for permeability screening.
  • A critical evaluation of the predictive power of current cell-based models is presented.
  • Limitations of high-throughput but less predictive models are highlighted.

Conclusions:

  • Cell-based models offer a balance between throughput and predictivity for intestinal permeability.
  • Future advancements in cell culture technology are expected to enhance predictive accuracy.
  • Continued development of cell models is vital for efficient drug discovery pipelines.