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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
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Published on: January 13, 2016

Use of microsatellite markers and gene dosage to quantify gene copy numbers in Candida albicans

J-M Costa1, O Eloy, F Botterel

  • 1Laboratoire de Parasitologie-Mycologie, Hôpital H. Mondor, 51 avenue du Maréchal DeLattre de Tassigny, 94010 Créteil, France.

Journal of Clinical Microbiology
|March 8, 2005
PubMed

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Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...