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Updated: May 1, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Sugars inhibit expression of the rugose phenotype of Vibrio cholerae
Afsar Ali1, J Glenn Morris, Judith A Johnson
1Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA. aali@epi.umaryland.edu
Abstract:
Vibrio cholerae can shift to a rugose colony phenotype, reflecting expression of an exopolysaccharide that provides protection against a variety of environmental stresses. Our data indicate that expression of the rugose phenotype is inhibited by a variety of sugars, including sucrose, dextrose, arabinose, fructose, and maltose. Inhibition by sucrose may be one factor in explaining the failure of rugose strains to grow on thiosulfate citrate bile salts sucrose agar, the primary selective medium for V. cholerae.
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