Related Experiment Video

Updated: Aug 19, 2026

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

[Antitumor effect and apoptosis induction in human cancer cell lines by BRM-SJS]

Zhao-Yang Liu1, Ze-Guang Wang, Xing-Jing Liu

  • 1Cancer Institute of Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R.China.

AI Zheng = Aizheng = Chinese Journal of Cancer
|March 9, 2005
PubMed
Abstract

Insights

BRM-SJS demonstrates significant antitumor activity against glioma, breast, and pancreas cancer cell lines in vitro. Its mechanism involves inducing apoptosis, characterized by cell shrinkage and DNA fragmentation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Context:

  • Clinical observations suggested BRM-SJS possesses antitumor properties.
  • This study aimed to validate these findings and elucidate the underlying mechanisms.

Purpose:

  • To investigate the in vitro antitumor activity of BRM-SJS.
  • To determine the mechanism of action, specifically focusing on apoptosis induction.

Summary:

  • BRM-SJS exhibited significant in vitro antitumor effects against human Suzhou human glioma (SHG-44), breast carcinoma (MCF-7), and human pancreas carcinoma (PANC1) cell lines.
  • Morphological changes, increased apoptosis (Annexin-V/PI flow cytometry), and DNA laddering (agarose gel electrophoresis) were observed in SHG-44 and MCF-7 cells treated with BRM-SJS.

Impact:

  • The findings suggest BRM-SJS has potential as an anticancer agent.
  • The study highlights apoptosis induction as a key mechanism of BRM-SJS's antitumor activity.

More Related Videos

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo09:19

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...