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Related Experiment Videos

Maltose utilization in Enterococcus faecalis.

Y Le Breton1, V Pichereau, N Sauvageot

  • 1Laboratoire de Microbiologie de l'Environnement, USC INRA, IRBA, Université de Caen, 14032 Caen Cedex, France.

Journal of Applied Microbiology
|March 9, 2005
PubMed
Summary

Enterococcus faecalis utilizes maltose via a phosphotransferase system (PTS) transporter, MalT, and subsequent hydrolysis by maltose phosphate phosphorylase (MalP). This research reveals a novel metabolic pathway for maltose in lactic acid bacteria.

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Area of Science:

  • Microbiology
  • Metabolic pathways
  • Bacterial genetics

Background:

  • Maltose is a key carbon source for many bacteria.
  • Understanding bacterial metabolic pathways is crucial for biotechnology and health.

Purpose of the Study:

  • To elucidate the metabolic pathway for maltose utilization in Enterococcus faecalis.
  • To identify the genes and proteins involved in maltose uptake and metabolism.

Main Methods:

  • Screening of Enterococcus faecalis insertional mutants to identify those impaired in maltose utilization.
  • Genetic analysis of insertion loci to identify relevant genes.
  • Analysis of radiolabeled carbohydrate uptake to determine transport mechanisms.

Main Results:

Related Experiment Videos

  • Mutants with insertions in malT (PTS transporter component) and malP (maltose phosphorylase homologue) were identified.
  • The malP gene is part of the malPBMR operon, including genes for phosphoglucomutase (malB), mutarotase (malM), and a transcriptional regulator (malR).
  • Over 97% of maltose uptake occurs via the MalT PTS transporter.

Conclusions:

  • Maltose enters Enterococcus faecalis via a PTS transporter (MalT), yielding maltose-6-phosphate intracellularly.
  • Maltose-6-phosphate is hydrolyzed by maltose phosphate phosphorylase (MalP).
  • This study describes a novel maltose utilization pathway in lactic acid bacteria.