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Related Experiment Videos

Carbapenemase-producing Enterobacteriaceae, U.S. rivers.

Cécile Aubron1, Laurent Poirel, Ronald J Ash

  • 1University Paris XI, Paris, France.

Emerging Infectious Diseases
|March 9, 2005
PubMed
Summary

Carbapenemase genes, specifically the inducible beta-lactamase IMI-2, were found in genetically identical Enterobacter asburiae from U.S. rivers. This indicates an environmental reservoir for these antibiotic resistance genes.

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Area of Science:

  • Environmental microbiology
  • Molecular biology
  • Antimicrobial resistance

Background:

  • Previous isolation of imipenem-resistant gram-negative rods from U.S. rivers.
  • Detection of imipenem hydrolysis in Enterobacter asburiae isolates.

Purpose of the Study:

  • To characterize the carbapenem resistance mechanism in Enterobacter asburiae isolates from U.S. rivers.
  • To investigate the genetic basis and transferability of the identified beta-lactamase.

Main Methods:

  • Random amplified polymorphism DNA analysis for genetic relatedness.
  • Identification and sequencing of beta-lactamase genes.
  • Plasmid analysis and DNA fragment sequencing.

Main Results:

Related Experiment Videos

  • Genetically indistinguishable Enterobacter asburiae isolates harbored an identical clavulanic acid-inhibited beta-lactamase, IMI-2.
  • The blaIMI-2 gene was located on a self-transferable 66-kb plasmid.
  • An upstream LysR-type regulator gene was identified, explaining the inducibility of IMI-2 expression.

    • Conclusions:

    • Beta-lactamase IMI-2 is the first identified inducible, plasmid-encoded carbapenemase.
    • Clonally related E. asburiae isolates from distant rivers suggest an environmental reservoir for carbapenemase genes.
    • The findings highlight the role of environmental bacteria in the dissemination of antibiotic resistance.