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A zucchini-peroxidase biosensor applied to dopamine determination.
Karina Omuro Lupetti1, Luiz Antônio Ramos, Iolanda Cruz Vieira
1Departamento de Química, Universidade Federal de São Carlos, São Carlos-SP, Brazil.
Farmaco (Societa Chimica Italiana : 1989)
|March 9, 2005
Summary
A novel biosensor using zucchini peroxidase effectively detects dopamine in pharmaceuticals. This method offers high accuracy and a low detection limit for dopamine analysis.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Biochemistry
Background:
- Dopamine determination is crucial in pharmaceutical analysis.
- Enzyme-based biosensors offer sensitive detection methods.
- Crude plant extracts can be a source of useful enzymes.
Purpose of the Study:
- To develop a biosensor for dopamine determination.
- To utilize peroxidase from zucchini (Cucurbita pepo) crude extract.
- To validate the biosensor for pharmaceutical samples.
Main Methods:
- Biosensor fabrication using zucchini peroxidase.
- Enzymatic catalysis of dopamine oxidation to dopaminequinone.
- Electrochemical detection of dopaminequinone reduction at -0.02 V.
Main Results:
- Dopamine recovery ranged from 94.8% to 106%.
- A linear analytical curve was obtained for dopamine concentrations between 5.0 x 10(-4) and 3.0 x 10(-3) mol L-1 (r=0.9982).
- The detection limit was 2.6x10(-5) mol L-1 with a relative standard deviation <1.2%.
Conclusions:
- The developed zucchini peroxidase biosensor is suitable for accurate dopamine quantification.
- The biosensor demonstrates good sensitivity and reproducibility.
- This method provides a viable approach for dopamine analysis in pharmaceutical formulations.