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Updated: Aug 19, 2026

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
Published on: March 10, 2021
Antibodies generated by a novel DNA vaccination identify the MHC class III encoded BAT2 polypeptide
Angelika Schneiders1, Stefan Thiel, Johannes Winkler
1Division of Immunobiology, Institute of Molecular Physiology, University of Bonn, Römerstrasse 164, 53117 Bonn, Germany.
Abstract:
Recombinant vaccines containing Ii sequences were employed to elicit an antibody response. Gene gun immunisation of mice with the recombinant Ii-antigen-encoding vectors induced antigen-specific antibodies. Antibody levels were substantially elevated when the DNA construct was extended by a sequence encoding the protease inhibitory domain of the invariant chain isoform Ii41. Employing this approach, we raised antibodies specific for a novel MHC class III encoded protein. The antibodies identify the 216 kDa BAT2 polypeptide. Immunostaining of embryonic tissue sections showed specific expression, especially in central nervous tissue, and suggests BAT2 as a novel differentiation marker.
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