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TILLING without a plough: a new method with applications for reverse genetics.

Erin J Gilchrist1, George W Haughn

  • 1Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, British Colombia, V6T 1Z4, Canada.

Current Opinion in Plant Biology
|March 9, 2005
PubMed
Summary
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Targeting Induced Local Lesions IN Genomes (TILLING) is a versatile reverse genetic method for detecting DNA variations. This high-throughput technique is applicable to diverse organisms and is widely used in plant science.

Area of Science:

  • Genetics
  • Molecular Biology

Background:

  • Reverse genetics enables the study of gene function by identifying mutations.
  • Existing methods have limitations regarding organism type, throughput, and genome characteristics.

Purpose of the Study:

  • To introduce and highlight the utility of Targeting Induced Local Lesions IN Genomes (TILLING) as a reverse genetic technique.
  • To emphasize the advantages of TILLING over other available methods.

Main Methods:

  • TILLING utilizes a mismatch-specific endonuclease.
  • This endonuclease detects induced or natural DNA polymorphisms.

Main Results:

  • TILLING is applicable to a wide range of organisms.
  • The technique is high-throughput and independent of genome size, reproductive system, or generation time.

Related Experiment Videos

  • TILLING is effectively used for detecting induced and natural genetic variation in plants.

    • Conclusions:

    • TILLING is a powerful and adaptable reverse genetic tool.
    • Its efficiency and broad applicability make it valuable for genetic variation studies, particularly in plant species.