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Related Experiment Videos

Vitamin E trafficking.

Maret G Traber1, Graham W Burton, Robert L Hamilton

  • 1Linus Pauling Institute, Oregon State University, Corvallis, OR 97331-6512, USA. maret.traber@oregonstate.edu

Annals of the New York Academy of Sciences
|March 9, 2005
PubMed
Summary
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The alpha-tocopherol transfer protein (alpha-TTP) is crucial for maintaining vitamin E levels. This study investigated how alpha-TTP enriches VLDL with alpha-tocopherol, finding it likely occurs post-secretion.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nutritional Science

Background:

  • The alpha-tocopherol transfer protein (alpha-TTP) is essential for preventing vitamin E deficiency.
  • alpha-TTP maintains alpha-tocopherol concentrations in plasma and tissues, but not intestinal absorption.
  • Impaired alpha-TTP function leads to vitamin E deficiency and affects very low-density lipoprotein (VLDL) secretion.

Purpose of the Study:

  • To elucidate the mechanism by which alpha-TTP incorporates alpha-tocopherol into nascent VLDLs.
  • To determine if alpha-TTP acts within the endoplasmic reticulum or Golgi apparatus during VLDL assembly.
  • To investigate the site of alpha-tocopherol enrichment in VLDL.

Main Methods:

  • Isolated nascent VLDLs from purified RER and Golgi apparatus membrane fractions in rats fed labeled alpha-tocopherols.

Related Experiment Videos

  • Analyzed deuterium-labeled RRR- and SRR-alpha-tocopherols in plasma and VLDL precursors.
  • Utilized liver perfusion studies with cynomolgus monkey livers.

    • Main Results:

    • VLDL particles secreted from the liver are preferentially enriched in RRR-alpha-tocopherol.
    • No enrichment of alpha-tocopherol was detected in VLDL precursors within the RER or Golgi apparatus.
    • Plasma showed enrichment of RRR-alpha-tocopherol, suggesting a post-secretory enrichment process.

    Conclusions:

    • Alpha-TTP's role in VLDL enrichment with alpha-tocopherol likely occurs after VLDL secretion, not during intracellular assembly.
    • A potential mechanism involves alpha-TTP facilitating alpha-tocopherol transfer to the hepatocyte plasma membrane.
    • Newly secreted VLDLs may acquire alpha-tocopherol and cholesterol in the space of Disse.