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Related Experiment Videos

The decrease in gamma-tocopherol in plasma and lipoprotein fractions levels off within two days of vitamin E

Isabella Sundl1, Ulrike Resch, Andreas R Bergmann

  • 1Human Nutrition & Metabolism Research and Training Center, Institute of Molecular Biosciences, Karl-Franzens University, Schubertstrasse 1, A-8010 Graz, Austria.

Annals of the New York Academy of Sciences
|March 9, 2005
PubMed
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High-dose vitamin E supplements increase alpha-tocopherol but decrease gamma-tocopherol in plasma. This vitamin E ratio stabilizes within 48 hours, with gamma-tocopherol better preserved in triglyceride-rich lipoproteins.

Area of Science:

  • Nutritional biochemistry
  • Human physiology
  • Lipid metabolism

Background:

  • Vitamin E encompasses several compounds, including alpha-tocopherol and gamma-tocopherol, with distinct biological roles.
  • Understanding the impact of vitamin E supplementation on tocopherol profiles is crucial for assessing its efficacy and safety.
  • Differential distribution of tocopherol isomers within lipoprotein fractions influences their physiological effects.

Purpose of the Study:

  • To investigate the dynamic changes in plasma and lipoprotein-associated alpha- and gamma-tocopherol concentrations following high-dose vitamin E supplementation.
  • To determine the time course of alterations in the gamma-/alpha-tocopherol ratio in various lipoprotein fractions.
  • To assess whether prolonged vitamin E supplementation beyond 48 hours leads to further changes in tocopherol concentrations.

Related Experiment Videos

Main Methods:

  • Five healthy volunteers received 1000 IU/day of RRR alpha-tocopherol for 4 days.
  • Plasma and lipoprotein fractions (VLDL, IDL, LDL, HDL) were analyzed for alpha- and gamma-tocopherol concentrations.
  • The ratio of gamma-tocopherol to alpha-tocopherol was calculated and compared to baseline levels over time.

Main Results:

  • Plasma alpha-tocopherol concentrations increased significantly during supplementation.
  • Plasma gamma-tocopherol concentrations decreased, leading to a significant reduction in the gamma-/alpha-tocopherol ratio starting from 48 hours.
  • The gamma-/alpha-tocopherol ratio was consistently higher in very-low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) compared to low-density lipoprotein (LDL) and high-density lipoprotein (HDL) after 12 hours.

Conclusions:

  • Vitamin E supplementation with RRR alpha-tocopherol alters the tocopherol balance, increasing alpha-tocopherol while decreasing gamma-tocopherol.
  • The observed decrease in gamma-tocopherol and the gamma-/alpha-tocopherol ratio appears to stabilize within 48 hours of supplementation.
  • Triglyceride-rich lipoproteins (VLDL and IDL) demonstrate a greater capacity for maintaining gamma-tocopherol compared to LDL and HDL, suggesting differential incorporation mechanisms.