Related Experiment Video
Updated: Jul 27, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Intra- and intercontinental molecular variability of an Alu insertion in the 3' untranslated region of the LDLR gene
A H Heller1, F M Salzano, R Barrantes
1Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil.
Abstract:
One-hundred three individuals from two Mongolian, two Siberian, and ten native American populations were studied in relation to a 340-bp sequence from an Alu insertion located in the 3' untranslated region of the LDLR gene. Seven haplotypes have been determined, and haplotype B1 was the most common, accounting for about half the sequences found. In general, diversity values are quite high, about 2.5 times higher than those found in other autosomal Alu sequences. Almost all (93%) of the variability occurs at the intrapopulation level, but the greatest among-group differentiation (6-8%) was found when we grouped in a single population all Native Americans plus Siberian Eskimos and Chukchi and compared them with Mongolians. This result is compatible with earlier mtDNA and Y-chromosome suggestions of a single origin for the first colonizers of the American continent. With this nuclear locus it was not possible to broadly distinguish between Central and South American natives. No evidence of selection or marked demographic changes was obtained with these data.
More Related Videos
07:26High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
Published on: July 18, 2017
08:46Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
Related Concept Videos
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Non-LTR Retrotransposons
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Single Nucleotide Polymorphisms-SNPs