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Updated: Aug 19, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
[Expression and biology identification of the human epididymis-specific gene ESC42 in E. coli]
Xiao-Fang Shen1, Jian-Yuan Li, Hai-Yan Wang
1Central Laboratory of Yantai Yuhuangding Hospital, Shandong Stem Cell Engineering Research Center, Yantai, Shandong 264000, China. sxfcym@yahoo.com.en
Objective:
To provide materials for the study of the function of ESC42 protein specifically expressed in the human epididymis.
Methods:
The ESC42 gene was amplified from the human epididymis cDNA library by PCR and then cloned into prokaryotic expression vector pGEX-4T-1, expressed and purified by recombinant DNA techniques. The specificity of ESC42 protein was identified by Western blot and MALDI-TOF-MS. The database was searched by Ms-Fit.
Results:
The recombinant plasmid expressed a Mr 38 x 10(3) fusion protein in E. coli at a level of 30% of the total protein, and the purity was as high as 99%. The ESC42 protein was identified by ESC42 monoclonal antibody and its molecular weight was 11 978.12, tested by MALDI-TOF-MS. The peptide mass fingerprint analysis showed that the coverage rate of the sequence reached 48% with 100% matching. The motif scan in Prosite database reveal that ESC42 belonged to the beta-defensin family and had antibacterial activity.
Conclusion:
Obtaining high purity of rhESC42 protein may lay a foundation for the study of its functions.
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