Dopamine modulates synaptic activity in the optic lobes of cuttlefish, Sepia officinalis

The effects of dopamine on spontaneous excitatory postsynaptic currents (sEPSCs) and inhibitory postsynaptic currents (sIPSCs) in three different classes of neurones within the optic lobe of cuttlefish were investigated using whole-cell voltage clamp techniques in a slice preparation. The neuronal types were centrifugal and amacrine neurones, located in the inner granular cell layer, and medullar interneurones, located within the central medulla of the optic lobes. The results demonstrate that bath application of dopamine (50 microM) reversibly reduced both the frequency and amplitude of sEPSCs and of sIPSCs in these optic lobe neurones. The inhibitory effects of DA were dose-dependent and neither D1- nor D2-like receptors appear to be implicated, but probably D4-like receptors are involved in these actions. By pre-applying tetrodotoxin (TTX, 0.5 microM), to block action potential-dependent EPSCs and IPSCs, it is shown that dopamine has no effect on the amplitude, frequency or decay time constant of the mEPSCs or mIPSCs. The results are the first to identify a specific physiological action of dopamine on cephalopod brain activity, they indicate that this effect is probably presynaptic to the specific classes of cells recorded from, and they provide information on the pharmacological profile of the receptors involved. The widespread inhibitory effect of dopamine on the activity of cuttlefish optic lobe neurones is discussed in the context of comparable data from vertebrate preparations and the actions of other neuromodulators in the cuttlefish brain.