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Related Experiment Videos

Computing numerically the access resistance of a pore.

Marcel Aguilella-Arzo1, Vicente M Aguilella, R S Eisenberg

  • 1Biophysics Unit, Department of Experimental Science, Universitat Jaume I, Castellón 12080, Spain.

European Biophysics Journal : EBJ
|March 10, 2005
PubMed
Summary

Access resistance (AR) calculations for ion channels differ based on membrane charge. Hall's equation overestimates AR in charged membranes, where it can be negligible, aiding conductance separation.

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Area of Science:

  • Biophysics
  • Computational Biology
  • Physical Chemistry

Background:

  • Access resistance (AR) is crucial for ion channel conductance, especially in wide, short channels.
  • Classical electrostatic models, like Hall's equation, are commonly used to estimate AR.
  • Discrepancies arise when applying these models to channels in charged environments.

Purpose of the Study:

  • To numerically calculate the access resistance (AR) of a channel at a circular pore entrance.
  • To compare numerical AR results with Hall's electrostatic equation.
  • To investigate the impact of charged membranes on AR and its relationship with pore radius.

Main Methods:

  • Solving the Poisson-Nernst-Planck equations numerically.
  • Simulating ion flow at the entrance of a circular pore model.

Related Experiment Videos

  • Comparing numerical AR values with analytical predictions from Hall's equation.

    • Main Results:

    • Numerical AR calculations agree with Hall's equation for uncharged pores in neutral membranes.
    • Hall's equation overestimates AR for channels in charged membranes.
    • AR is significantly lower in charged membranes and can be negligible under certain conditions.
    • AR shows weak dependence on pore radius in charged membranes at low salt concentrations.

    Conclusions:

    • Hall's electrostatic model is accurate for uncharged channels but overestimates AR in charged membranes.
    • Numerical methods provide a more accurate AR assessment for channels in charged environments.
    • The distinct behavior of AR in charged membranes allows for the separation of channel and access resistance contributions to conductance.