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Expression of ADAM15 in lung carcinomas.
1Institute of Pathology, University of Leipzig, Liebigstrasse 26, 04103, Leipzig, Germany. schuetza@medizin.uni-leipzig.de
Virchows Archiv : an International Journal of Pathology
|March 10, 2005
Summary
ADAM15 expression is frequent in lung carcinoma cell lines and tissues, often co-localizing with integrin alpha(v)beta3. This suggests a role for ADAM15 in lung cancer progression and metastasis.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- ADAM15, a metalloprotease, has domains suggesting involvement in tumor invasion and metastasis.
- Integrin alpha(v)beta3 is a potential ligand for ADAM15.
Purpose of the Study:
- To analyze ADAM15 and integrin alpha(v)beta3 expression in lung carcinoma cell lines and tissues.
- To investigate the correlation between ADAM15 expression and clinicopathological features.
Main Methods:
- Analysis of mRNA expression in lung carcinoma cell lines.
- Flow cytometry to detect cell surface expression of alpha(v)beta3 heterodimer.
- Immunohistochemical staining of tumor tissues for ADAM15 and alpha(v)beta3 integrin.
Main Results:
- Most small cell lung carcinoma (SCLC) and non-SCLC cell lines showed positive mRNA expression for ADAM15 and integrin subunits.
- Adenocarcinomas (ACs) exhibited the strongest ADAM15 staining, while SCLCs showed weak expression.
- ADAM15 expression was often higher in invasive tumor cells and frequently co-localized with alpha(v)beta3 integrin.
Conclusions:
- Lung carcinoma cell lines and tissues frequently express ADAM15.
- ADAM15 and integrin alpha(v)beta3 are often co-expressed in lung carcinomas.
- ADAM15 expression does not significantly correlate with tumor stage or differentiation.