Single neuron activity changes to interleukin-1beta in the orbitofrontal cortex of the rat

The orbitofrontal cortex (OBF) is known to play important roles in various regulatory processes. Our preliminary behavioral studies showed homeostatic alterations after orbitofrontal cortical microinjection of interleukin-1beta (IL-1beta) in the rat. To elucidate whether the above alterations were due to direct neuronal action of the cytokine, extracellular single neuron activity was recorded in the OBF of anesthetized rats by means of tungsten fiber multibarreled glass microelectrodes during microelectrophoretic administration of IL-1beta. More than half (56%) of all cells tested changed in firing rate in response to IL-1beta. Approximately 90% of these cytokine-modulated neurons were also sensitive to microelectrophoretically applied d-glucose, that is, proved to be the elements of the central glucose-monitoring neural network. The present findings demonstrate that IL-1beta can exert direct modulatory role on neurons in the OBF.