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Updated: Aug 19, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Comparative single-turnover kinetic analyses of trans-cleaving hammerhead ribozymes with naturally derived
Marc S Weinberg1, John J Rossi
1Division of Molecular Biology, Beckman Research Institute of the City of Hope, Graduate School of Biological Sciences, Duarte, CA 91010, USA.
Abstract:
trans-Cleaving hammerhead ribozyme variants were generated with mimicked non-conserved internal loop motifs derived from five structurally diverse natural cis-cleaving ribozymes. Most modified trans-cleaving variants showed enhanced single-turnover cleavage rates relative to minimal counterparts that lack tertiary interactions between internal loop motifs I and II, and relative to controls with sequence changes in loop I. The trans-cleaving ribozyme derived from the positive strand of peach latent mosaic viroid had the highest observed cleavage rate, suggesting a structurally optimized motif that facilitates rapid formation of the ribozyme catalytic center in a trans-reaction.
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