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Distinction in gene expression profiles demonstrated in parathyroid adenomas by high-density oligoarray technology
Lars Forsberg1, Erik Björck, Jamileh Hashemi
1Department of Molecular Medicine, Karolinska University Hospital CMM L8:01, SE-17176 Stockholm, Sweden.
European Journal of Endocrinology
|March 11, 2005
Summary
Somatic deletion of chromosome 11q13 is common in parathyroid adenomas. Gene expression profiling reveals distinct profiles in tumors with and without 11q13 loss of heterozygosity (LOH), suggesting a role in tumor development.
Area of Science:
- Endocrinology
- Oncology
- Genetics
Background:
- Somatic deletion of chromosome 11q13 is the most frequent genetic aberration in parathyroid adenoma.
- Understanding the genetic underpinnings of parathyroid tumor development is crucial.
Purpose of the Study:
- To investigate the gene expression profiles of parathyroid adenomas and normal parathyroid tissues.
- To evaluate gene expression differences in relation to 11q13 loss of heterozygosity (LOH).
Main Methods:
- Gene expression profiling using Affymetrix HG-U95Av2 oligonucleotide arrays.
- Quantitative real-time PCR (QRT-PCR) for validation.
- Unsupervised hierarchical clustering and t-test analysis to compare tumor subgroups.
Main Results:
- Cyclin D1 and c-Jun showed increased expression in adenomas compared to normal tissues.
- Parathyroid adenomas clustered into two groups based on 11q13 LOH status.
- The oncogene ENC 1 was highly overexpressed in tumors with 11q13 LOH.
Conclusions:
- Microarray analyses revealed distinct and common expression profiles in parathyroid adenomas with and without 11q13 LOH.
- Approximately half of the underexpressed genes were mapped to chromosome 11, supporting a dose effect.
- These findings provide insights into the genetic etiology of parathyroid tumor development.