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Detection of chromosomal abnormalities by comparative genomic hybridization
Jean-Michel Lapierre1, Gérard Tachdjian
1Service d'Histologie-Embryologie-Cytogénétique, Hôpital Necker-Enfants Malades, Paris bService de Biologie du Développement et de la Reproduction-Cytogénétique, Hôpital Antoine Béclère, Clamart, France. jm_lapierre@yahoo.fr
Purpose Of Review:
Comparative genomic hybridization (CGH) is a modified in-situ hybridization technique. In this type of analysis, two differentially labeled genomic DNAs (study and reference) are cohybridized to normal metaphase spreads or to microarray. Chromosomal locations of copy number changes in the DNA segments of the study genome are revealed by a variable fluorescence intensity ratio along each target chromosome. Thus, CGH allows detection and mapping of DNA sequence copy differences between two genomes in a single experiment.
Recent Findings:
Since its development, comparative genomic hybridization has been applied mostly as a research tool in the field of cancer cytogenetics to identify genetic changes in many previously unknown regions. It is also a powerful tool for detection and identification of unbalanced chromosomal abnormalities in prenatal, postnatal and preimplantation diagnostics.
Summary:
The development of comparative genomic hybridization and increase in resolution analysis by using the microarray-based technique offer new information on chromosomal pathologies and thus better management of patients.