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Updated: Aug 14, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
Published on: October 13, 2009
The development of an algorithm for the mass spectral interpretation of phosphoproteins
1Department of Chemical Engineering, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Abstract:
Extended from the peptide mapping method (Fenyo, D., Curr. Opin. Biotechnol. 2000, 11, 391-395) the proposed algorithm takes the mass information of a precursor ion to reconstruct all possible phosphorylated peptide sequences. The mass spectra of product ions from the corresponding precursor ion is then used and compared to the reconstructed sequences to deduce the most probable phosphoprotein. The proposed algorithm also predicts all possible combinations of phosphopeptides, which may serve as a clue for designing proper phosphorylation experiments to validate the existence of these peptides and the corresponding proteins.
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