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Efficacy testing of disinfectants.

Stephen F Tomasino¹

¹U.S. Environmental Protection Agency, Environmental Science Center, OPP Microbiology Laboratory, 701 Mapes Rd, Ft. Meade, MD 20755-5350, USA. Tomasino.Stephen@epa.gov

Journal of AOAC International

|March 12, 2005

Summary

The U.S. Environmental Protection Agency is improving sporicidal efficacy test methods for decontamination. Research focuses on quantitative methods and refining the AOAC Sporicidal Activity Test for better performance verification.

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Area of Science:

Environmental Science
Microbiology
Chemical Efficacy Testing

Background:

The U.S. Environmental Protection Agency's (EPA) Office of Pesticide Programs (OPP) regulates antimicrobial products, including sporicides.
Following the 2001 anthrax attacks, EPA initiated research to enhance the performance verification of sporicides for building decontamination.
The OPP Microbiology Laboratory leads this research effort.

Purpose of the Study:

To evaluate and improve existing efficacy test methods for sporicides.
To develop and test modifications for the AOAC Sporicidal Activity Test (AOAC Method 966.04).
To establish quantitative methodologies for assessing sporicidal efficacy.

Main Methods:

Evaluating quantitative methods for sporicidal efficacy assessment.
Developing and comparatively testing modifications to the AOAC Sporicidal Activity Test (AOAC Method 966.04).
Planning future studies on Bacillus anthracis surrogates and multilaboratory validation.

Main Results:

Research is actively evaluating quantitative methodologies for sporicidal efficacy.
Modifications to the AOAC Sporicidal Activity Test are under development and comparative testing.
Preliminary data and general conclusions are presented in the report.

Conclusions:

Ongoing research aims to improve the reliability and accuracy of sporicidal efficacy testing.
Enhanced test methods are crucial for ensuring effective decontamination against spore-forming pathogens.
Future validation studies will further refine quantitative methods and surrogate use.

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