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Characterization of two galactosidases extracted from wheat germ with a hydroalcoholic solvent
M P Papet1, D Delay, M Monsigny
1Département de Biochimie des Glycoconjugués et des Lectines Endogènes, CNRS, Orléans, France.
Biochimie
|January 1, 1992
Abstract:
Alpha- and beta-D-galactosidases were characterized from a hydroalcoholic extract of wheat germ (Triticum vulgare). Kinetic constants (Vmax and KM) and the optimal pHs for the hydrolysis of p-nitrophenyl galactopyranosides by both enzymes were determined. These enzymes presented a high stability in hydroalcoholic medium and were inhibited by iodoacetamide and sodium p-hydroxy-mercuribenzoate.