Some basic parameters of microspheres fabricated from a branched oligoester by a rapid procedure

Microspheres were prepared from a branched copolymer of DL-lactic acid with mannitol containing native albumin and albumin labelled with fluorescein isothiocyanate, using a rapid method of distribution of methylformate as the solvent of the copolymer from the intermediate phase of the multiple w/o/w emulsion. The primary w/o emulsion was prepared by the method of homogenization with a turbine or, alternatively, by the method of dispersion with ultrasound in modified vessels. Different additives in the external aqueous phase, such as polyvinyl alcohol or the gelatin hydrolyzate as emulsifiers were tested. Ammonium sulphate, methylformate or ethyl acetate were used as moderators of solidification of microspheres. The effect of these selected formulation parameters on the size, encapsulation efficiency, yield of microspheres and on the course of the BSA and FITC-BSA release in vitro conditions were examined.