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[Establishment of reference values for fructosamine and HbA1c in childhood]
G Wiedemann1, T Simon, U Hildebrand
1Klinisch-Chemisches Laboratorium, Medizinischen Akademie, Erfurt.
Insights
Establishing reference ranges for fructosamine and glycated hemoglobin (HbA1c) in children is crucial. This study provides age-specific 95% intervals for these markers in a pediatric cohort aged 1 day to 9 years.
Area of Science:
- Pediatric Endocrinology
- Clinical Chemistry
- Laboratory Medicine
Background:
- Lack of established reference values for fructosamine and glycated hemoglobin (HbA1c) in pediatric populations.
- Need for reliable biochemical markers to monitor glycemic control in children.
Purpose of the Study:
- To determine age-specific reference values for fructosamine and HbA1c in children.
- To establish 95% confidence intervals for these markers across different pediatric age groups.
Main Methods:
- Analysis of plasma samples from 522 children aged 1 day to 9 years.
- Determination of fructosamine, total protein, glucose, and HbA1c using plasma electrophoresis and blood analysis.
- Statistical analysis including Mann-Whitney-U-test and calculation of 95% intervals for various age strata.
Main Results:
- Age-stratified reference intervals were calculated for fructosamine, protein-corrected fructosamine, and HbA1c.
- Significant differences in values were observed between different pediatric age groups.
- Established 95% intervals provide a basis for interpreting these markers in children.
Conclusions:
- This study provides essential age-specific reference ranges for fructosamine and HbA1c in children.
- These reference values will aid in the accurate assessment of glycemic status and long-term glucose control in pediatric patients.
- The findings support improved clinical decision-making for diabetes management in children.
Abstract:
Reference values for fructosamine and glycated haemoglobin in children are still lacking. Using samples from 522 children (age range 1 day to 9 years), plasma electrophoresis was performed, fructosamine and total protein were determined in EDTA-plasma, and glucose and the fraction of glycated haemoglobin (HbA1c) were determined in EDTA blood. The results were grouped according to age (1, 3 and 5 days, 6-28 days, 2-12 months, 2-3 years, 4-6 years, and 7-9 years). Differences between the age groups were determined using the Mann-Whitney-U-test, and the values in each group were summarized where possible. The 95%-interval was calculated for fructosamine, protein-corrected fructosamine, and HbA1c.